Research Topics / Cell cycle
   
The proper control of cell cycle is essential  for the proliferation of cells, malfunction has severe consequences such as tumor development. купить подушку Киев
   
Life is based on cellular replication. For faithful inheritance and maintenance of genetic identity, exact replication of the genome and its accurate segregation into the dividing daughter cells are mandatory. Cellular replication is controlled by the cell division cycle, a process that responds to extracellular cues and results in two genetically identical daughter cells. The cell cycle is divided into the actual division or M-phase, including mitosis (nuclear division) and cytokinesis (cellular division), as well as interphase, which consists of G1-, S- and G2-phase. In all eukaryotes transitions between these phases are tightly controlled by cyclin dependent kinases (CDKs). Defects at various stages of the cell cycle contribute to cancer. Deregulation of the G1-phase is the most common cause of tumorigenesis. Errors in DNA replication (S-phase) and chromosome segregation during mitosis cause aneuploidy, a characteristic hall mark of all cancer.
   
Cell cycle research in Innsbruck

Cell cycle research in Innsbruck focuses on the regulation of G1-phase by CDK inhibitors (CKI), the ubiquitin ligase APC/C (anaphase promoting complex) during interphasem and the mechanisms that control chromosome segregation during mitosis as well as cytokinesis.
CKIs are proteins that can interact with different classes of CDKs to constrain their activity. Loss of CKI function contributes to tumorigenesis. One of our aims is to understand how CKIs are controlled in normal and tumor cells.
Ubiquitin-dependent proteolysis is a key regulatory event of the cell division cycle. Two multi-protein main ubiquitin ligases, the anaphase promoting complex APC/C and the SCF control the abundance of major cell cycle regulators and, thus, cell cycle progression. One major aim of our works is to understand the role of the APC/C in interphase cells.
   
Teams

   
PhD Training

Within MCBO we offer several lectures on basic concepts of cell cycle regulation as well as hands-on courses, including live cell imaging, production of recombinant kinases, advanced DNA cloning technologies and others.
   
Technologies

Recombinant protein expression in bacteria and insect cells; retro-, lenti-, adenoviral gene expression technology; gene targeting in murine ES cells and in human cells using AAV-technology; recombineering; genome editing using TALEN technology; transgenic inducible RNAi; vertebrate model organisms (mouse and zebrafish); live cell imaging; FRET-, FRAP-, TIRF-microscopy.