Spiegl Simon,

University / Clinic: Medical University Innsbruck
Institute: Division of Molecular Pathophysiology
 
Research Area: Cell Cycle Control / Leukemia Apoptosis

Email: simon.spiegl@i-med.ac.at
Web: http://www.tkfi.at/dmp/

  Funded by: MUI

Research Topic:
Validation and functional analysis of candidate genes identified by expression profiling and proteomics

‘OMICS’ technologies have become a powerful tool to elucidate cellular pathways. Typically these approaches lead to the generation of candidate gene lists that rank the identified genes or proteins according to their expression levels, change of regulation or modification. Bioinformatic approaches often extract information from these sets of co-regulated genes, even if the exact function of each particular gene is unknown. To derive hypotheses concerning the role of certain genes in a pathway biochemical and cell biological assays are required to experimentally test these candidate genes. Due to the high number of candidate genes obtained by ‘OMICS’ technologies and the time- and cost-intensive nature of conventional assays, new technologies have to be developed or optimized to allow a rapid screening of candidate genes.
In this project we will develop assays to test the role of candidate genes identified by expression profiling as well a candidate phosphoproteins identified by proteomics with the aim to arrive at a more complete understanding of glucocorticoid-induced cell death in human leukemia as well as the identification of new players in WNT signaling in cancer. Carlo Pazolini
Карло Пазолини

Selected publications:
 
  Schuler F, Weiss JG, Lindner SE, Lohmüller M, Herzog S, Spiegl SF, Menke P, Geley S, Labi V, Villunger A
Checkpoint kinase 1 is essential for normal B cell development and lymphomagenesis
Nat Commun. 2017 Nov 22;8(1):1697. doi: 10.1038/s41467-017-01850-4. PubMed PMID: 29167438; PubMed Ce