Troppmair Jakob, Univ.-Prof. Mag.Dr.rer.nat.

University / Clinic: Innsbruck Medical University
Institute: Daniel-Swarovski Research Laboratory (DSL) Department of Visceral- Transplant- and Thoracic Surgery
Research Area: Molecular oncology, apoptosis, cell survival control, transplantation biology, ischemia, reperfusion


Research Topic:
The goal of our work is to understand cellular signaling and its role in the determination of cell fate in terms of proliferation, survival, etc. under normal and patho-physiological conditions.

Our interest lies in the dissection of intracellular signaling pathways and of the mechanism controlling signal propagation along them. Historically, this work focused on mitogen- activated protein kinases (MAPKs), but other signaling entities (NF-KB, PI-3-kinase, protein kinase B) have also been studied. Of particular interest to us is to use this knowledge to control aberrant signaling in diseases such as cancer, ischemie/reperfusion injury or neurodegeneration.

Ongoing research projects:
-Analysis of mechanisms underlying the survival control by Raf and PKB
-Regulation of ischemia/reperfusion (I/R) induced tissue damage by MAPKs
-control of mitochondrial function by intracellular signaling
-cellular stress signaling
-Role of Raf and Raf effector pathways in malignant transformation
-Stress kinase signaling in transformation

  Role of MAPKs and PKC in the activation of p66SHC
details ...

Selected publications:
  Haller M, Khalid S, Kremser L, Fresser F, Furlan T, Hermann M, Guenther J, Drasche A, Leitges M, Giorgio M, Baier G, Lindner H, Troppmair J
Novel insights into the PKCβ-dependent regulation of the oxidoreductase p66Shc.
J Biol Chem. 2016 Sep 13. pii: jbc.M116.752766. [Epub ahead of print] PubMed PMID: 27624939.

  Khalid S, Drasche A, Thurner M, Hermann M, Ashraf M, Fresser F, Baier G, Kremser L, Lindner H, Troppmair J
cJun N-terminal kinase (JNK) phosphorylation of serine 36 is critical for p66Shc activation.
Sci Rep. 2016 Feb 12;6:20930. doi: 10.1038/srep20930. PubMed PMID: 26868434; PubMed Central PMCID: P

  Enthammer M, Papadakis E, Salomé Gachet M, Deutsch M, Schwaiger S, Koziel K, Ashraf M, Khalid S, Wolber G, Packham G, Cutress R, Stuppner H, Troppmair J
Isolation of a novel thioflavin S-derived compound that inhibits BAG-1-mediated protein interactions and targets BRAF inhibitor-resistant cell lines.
Mol Cancer Ther. 2013 Nov;12(11):2400-14. doi: 10.1158/1535-7163.MCT-13-0142. Epub 2013 Sep 18. PubM

  Koziel K, Smigelskaite J, Drasche A, Enthammer M, Ashraf M, Khalid S, Troppmair J

RAF and antioxidants prevent cell death induction after growth factor abrogation through regulation of Bcl-2 proteins.
Exp Cell Res. 2013 Oct 15;319(17):2728-38. doi: 10.1016/j.yexcr.2013.07.029. Epub 2013 Aug 7. PubMed

  Zebisch A, Wölfler A, Fried I, Wolf O, Lind K, Bodner C, Haller M, Drasche A, Pirkebner D, Matallanas D, Rath O, Blyth K, Delwel R, Taskesen E, Quehenberger F, Kolch W, Troppmair J, Sill H
Frequent loss of RAF kinase inhibitor protein expression in acute myeloid leukemia.
Leukemia. 2012 Aug;26(8):1842-9. doi: 10.1038/leu.2012.61. Epub 2012 Mar 5. PubMed PMID: 22388727.

  Filipiak W, Sponring A, Filipiak A, Ager C, Schubert J, Miekisch W, Amann A, Troppmair J
TD-GC-MS analysis of volatile metabolites of human lung cancer and
normal cells in vitro
Cancer Epidemiol Biomarkers Prev. 2010 Jan;19(1):182-195.

  Kuznetsov A, Hermann M, Troppmair J, Margreiter R, Hengster P
Complex patterns of mitochondrial dynamics in human pancreatic cells revealed by fluorescent confocal imaging
J Cell Mol Med. 2010 Jan;14(1-2):417-425.

  Zebisch A, Haller M, Hiden K, Goebel T, Hoefler G, Troppmair J, Sill H.
Loss of RAF kinase inhibitor protein is a somatic event in the pathogenesis of therapy-related acute myeloid leukemias with C-RAF germline mutations.
Leukemia. 2009 Jun;23(6):1049-1053.

  Houben R, Ortmann S, Drasche A, Troppmair J, Herold M, Becker J
Proliferation arrest in B-Raf mutant melanoma cell lines upon MAPK pathway activation.
J Invest Dermatol. 2009 Feb;129(2):406-414.

  Hermann M, Kuznetsov A, Maglione M, Smigelskaite J, Margreiter R, Troppmair J
Cytoplasmic signaling in the control of mitochondrial uproar?
Cell Commun Signal. 2008 Aug 19;6:4.

  Kuznetsov A, Smigelskaite J, Doblander C, Janakiraman M, Hermann M, Wurm M, Scheidl S, Sucher R, Deutschmann A, Troppmair J
Survival signaling by C-RAF: mitochondrial reactive oxygen species and Ca2+ are critical targets.
Mol Cell Biol. 2008 Apr;28(7):2304-13.

  Fischer S, Weishaupt A, Troppmair J, Martini R
Increase of MCP-1 (CCL2) in myelin mutant Schwann cells is mediated by MEK-ERK signaling pathway
Glia 2008 Jun;56(8):836-843.

  McCubrey J, Abrams S, Ligresti G, Misaghian N, Wong E, Steelman L, Bäsecke J, Troppmair J, Libra M, Nicoletti F, Molton S, McMahon M, Evangelisti C, Martelli A
Involvement of p53 and Raf/MEK/ERK pathways in hematopoietic drug resistance
Leukemia 2008 Nov;22(11):2080-2090.

  Zebisch A, Czernilofsky A, Keri G, Smigelskaite J, Sill H, Troppmair J
Signaling through RAS-RAF-MEK-ERK: from basics to bedside.
Curr Med Chem. 2007;14(5):601-23.

  Aigner F, Maier H, Schwelberger H, Wallnöfer E, Amberger A, Obrist P, Berger T, Mak T, Maglione M, Margreiter R, Schneeberger S, Troppmair J
Lipocalin-2 regulates the inflammatory response during ischemia and reperfusion of the transplanted heart.
Am J Transplant. 2007 Apr;7(4):779-788.

  Zebisch A, Troppmair J
Back to the roots: the remarkable RAF oncogene story
Cell Mol Life Sci. 2006 Jun;63(11):1314-1330.

  Zebisch A, Staber P, Delavar A, Bodner C, Hiden K, Fischereder K, Janakiraman M, Linkesch W, Auner H, Emberger W, Windpassinger C, Schimek M,
Höfler G, Troppmair J, Sill H
Two transforming C-RAF germ-line mutations identified in patients with therapy-related acute myeloid leukemia

Cancer Res. 2006 Apr 1;66(7):3401-3408.

  Rennefahrt U, Janakiraman M, Ollinger R, Troppmair J
Stress kinase signaling in cancer: fact or fiction?
Cancer Lett. 2005 Jan 10;217(1):1-9.

  Götz R, Wiese S, Takayama S, Camarero G, Rossoll W, Schweizer U, Troppmair J, Jablonka S, Holtmann B, Reed J, Rapp U, Sendtner M
Bag1 is essential for differentiation and survival of hematopoietic and neuronal cells
Nat Neurosci. 2005 Sep;8(9):1169-1178.

  Kuznetsov A, Janakiraman M, Margreiter R, Troppmair J
Regulating cell survival by controlling cellular energy production: novel functions for ancient signaling pathways?
FEBS Lett. 2004 Nov 5;577(1-2):1-4.